Journal
RESEARCH IN MICROBIOLOGY
Volume 159, Issue 7-8, Pages 557-561Publisher
ELSEVIER
DOI: 10.1016/j.resmic.2008.06.006
Keywords
Biofilm; Extracellular polymeric substances; Acidithiobacillus ferrooxidans; Raman spectroscopy; Atomic force microscopy
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Funding
- Council of Scientific and Industrial Research (CSIR), New Delhi, India
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Acidithiobacillus ferrooxidans biofilm grown on uranium ore substrate was analyzed by a micro-Raman spectrometer and an atomic force microscope (AFM). The bacterium employed for this study, A. ferrooxidans BMI, was isolated from a uranium mine (Jaduguda, India). Micro-Raman analysis revealed the different constituents of molecular fragments present in microbial cells and in secreted extracellular polymeric substances (EPSs). AFM images clearly revealed bacterial cells surrounded by EPS. From Raman spectral data, the composition of EPS from A. ferrooxidans BMI appeared to be similar to that of EPS secreted in a different Pseudomonas bacterium. (C) 2008 Elsevier Masson SAS. All rights reserved.
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