Journal
REPRODUCTIVE BIOMEDICINE ONLINE
Volume 28, Issue 4, Pages 475-484Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rbmo.2013.11.014
Keywords
cell cycle; early fertilization events; known implantation; pronuclei; S-phase; time-lapse
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The morphology of fertilization events has been related to successful implantation by subjective criteria (pronuclei score, pronuclei symmetry and position). This work first described these events by time-lapse technology and then compared the timings of fertilization events (second polar body extrusion, first and second pronuclei appearance, abuttal and fading) in implanted versus nonimplanted embryos in a 2-year cohort retrospective study. A total of 1448 transferred embryos from 842 patients undergoing intracytoplasmic sperm injection with oocyte donation were monitored, 212 embryos from treatments where the number of gestational sacs matched the number of transferred embryos and 687 embryos from treatments no biochemical pregnancy was achieved. The timings at which second polar body extrusion (3.3-10.6 h), pronuclear fading (22.2-25.9 h) and length of S-phase (5.7-13.8 h) occurred were linked successfully to embryo implantation. The other parameters were apparently not related, as determined by image acquisition and time-lapse analysis. (C) 2013, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
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