Journal
REPRODUCTIVE BIOMEDICINE ONLINE
Volume 26, Issue 1, Pages 22-29Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rbmo.2012.10.007
Keywords
culture media; in-vitro maturation; IVF; melatonin; oocyte maturation; PCOS
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Funding
- Korea Healthcare Technology R and D Project, Ministry for Health and Welfare, Republic of Korea [A084923]
- Korea Health Promotion Institute [A084923] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Human pre-ovulatory follicular fluid (FF) contains a higher concentration of melatonin than serum. The aim of this study was to evaluate the effect of melatonin supplementation of culture medium on the clinical outcomes of an in-vitro maturation (IVM) IVF-embryo transfer programme for patients with polycystic ovarian syndrome (PCOS). Melatonin concentrations in the culture media of granulosa cells (GC) or cumulus-oocyte-complexes (COC) were measured and the clinical outcomes after using IVM media with or without melatonin were analysed. In the culture media of GC or COC, melatonin concentrations gradually increased. When human chorionic gonadotrophin priming protocols were used, implantation rates in the melatonin-supplemented group were higher than those of the non-supplemented control group (P < 0.05). Pregnancy rates were also higher, although not significantly. The findings suggest that the addition of melatonin to IVM media may improve the cytoplasmic maturation of human immature oocytes and subsequent clinical outcomes. It is speculated that follicular melatonin may be released from luteinizing GC during late folliculo-genesis and that melatonin supplementation may be used to improve the clinical outcomes of IVM IVF-embryo transfer. RBMOnline (C) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
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