Journal
REPRODUCTION IN DOMESTIC ANIMALS
Volume 47, Issue 6, Pages 959-964Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1439-0531.2012.01999.x
Keywords
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Funding
- CICYT [AGL2006-07769/GAN]
- European Social Fund (Madrid, Spain)
- Generalitat Valenciana (Spain) [GV/2007/163]
- Ministerio de Ciencia e Innovacion, Madrid, Spain [BES-2007-17036]
- Conselleria d'Agricultura, Pesca y Alimentacio (DOGV), CAPA, Valencia, Spain [5324]
- INIA-CCAA
- European Social Fund [DR03-704]
- Ministerio de Ciencia e Innovacion
- Subprograma Ramon y Cajal
- European Social Fund, Madrid, Spain [RYC-2010-06162]
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Cryopreserved boar sperm is not used extensively for artificial insemination, owing to the poor fertility rates of the sperm after freezing and thawing. The sperm membrane is damaged as the cells are cooled from body temperature to 5 degrees C (cold shock), as well as during the freezethaw process. Increasing the cholesterol content of boar sperm membranes could help them survive cryopreservation, similar to sperm from other species that are cold shock sensitive. The aim of this study was to determine the optimal cholesterol-loaded cyclodextrin (CLC) concentration to use for boar sperm cryopreservation, and the influence of CLCs on the cryosurvival of sperm from boars classified as good or poor freezers. Treating boar sperm with 1 mg of CLC/120 x 10(6)sperm slightly improved (p < 0.05) the percentage of viable sperm after freezingthawing. On the other hand, sperm, from both good and poor freezers, responded similarly to CLC treatment. Nevertheless, additional studies will be needed to study the effect of this treatment on other parameters of sperm quality.
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