Journal
REPRODUCTION FERTILITY AND DEVELOPMENT
Volume 24, Issue 8, Pages 1048-1054Publisher
CSIRO PUBLISHING
DOI: 10.1071/RD12002
Keywords
antioxidants; in vitro fertilisation; oxidative stress; pigs; polyspermy
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Funding
- Virginia Academy of Science
- Thomas F. Jeffress and Kate Miller Jeffress Memorial Trust
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The effects of supplementation with 1.5mM N-acetyl-L-cysteine (NAC) during in vitro oocyte maturation were studied. Oocytes were supplemented with 1.5mM NAC during maturation for 0 to 24 h, 24 to 48 h, or 0 to 48 h then subjected to IVF and embryo development. Oocytes were evaluated after maturation for intracellular glutathione concentration, superoxide dismutase and glutathione peroxidase activities and DNA fragmentation. Fertilisation and embryonic development success were also evaluated. There was no effect of treatment on intracellular glutathione concentrations, enzyme activities or fertilisation success rates. Supplementing NAC during maturation significantly decreased (P < 0.05) the percentage of oocytes with fragmented DNA compared with no NAC supplementation. Supplementing NAC from 24 to 48 h or 0 to 48 h resulted in a significantly higher (P < 0.05) percentage of oocytes with male pronuclei than for oocytes from the other treatment groups. There was no difference in the percentage of embryos cleaved by 48 h after IVF between treatment groups. Supplementing NAC from 24 to 48 h or 0 to 48 h resulted in a significantly higher (P < 0.05) percentage of embryos reaching the blastocyst stage by 144 h after IVF compared with the other treatment groups. These results indicate that supplementation of the oocyte maturation medium with 1.5mM NAC, specifically during the last 24 h, improves male pronucleus formation and blastocyst development in pigs.
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