Journal
REPRODUCTION
Volume 144, Issue 1, Pages 101-109Publisher
BIOSCIENTIFICA LTD
DOI: 10.1530/REP-11-0268
Keywords
-
Categories
Funding
- DGAPA/UNAM [IN211907, IN221110, IN211809]
- DGAPA/IXTLI [IX200910]
- CONACyT-Mexico [56660, 49113, 128566]
Ask authors/readers for more resources
Progesterone is a physiological agonist for mammalian sperm, modulating its flagellar movement and facilitating the acrosome reaction. To study the initial action of progesterone, we developed a caged analog with a photosensitive group: nitrophenylethanediol, at position 20. Using this compound combined with stroboscopic illumination, we performed Ca2+ imaging of human spermatozoa and analyzed the effects of progesterone on the intracellular Ca2+ concentration ([Ca2+](i)) of beating flagella for the first time. We observed a transient [Ca2+](i) increase in the head and the flagellum upon photolysis of the caged progesterone and an increase in flagellar curvature. Detailed kinetic analysis revealed that progesterone elicits an increase in the [Ca2+](i) immediately in the flagellum (mid-piece and principal piece), thereafter in the head with a short time lag. This observation is different from the progesterone-induced Ca2+ mobilization in mouse spermatozoa, where the Ca2+ rise initiates at the base of the sperm head. Our finding is mostly consistent with the recent discovery that progesterone activates CatSper channels in human spermatozoa, but not in mouse spermatozoa. Reproduction (2012) 144 101-109
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available