Journal
REGENERATIVE MEDICINE
Volume 9, Issue 4, Pages 437-452Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/RME.14.26
Keywords
cell therapy; human-specific biomarker; image quantification; stem cells; xenotransplantation
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Funding
- Thierry Latran Foundation
- INSERM
- Institut Pasteur
- NIH [1R01NS079702]
- Craig H. Neilsen Foundation [190140]
- Televie program of the 'Fonds National de la Recherche Scientifique' (FNRS, Brussels, Belgium)
- Fonds Yvonne Boel
- European Regional Development Fund
- Walloon Region
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Aim: Biomarker-based tracking of human stem cells xenotransplanted into animal models is crucial for studying their fate in the field of cell therapy or tumor xenografting. Materials & methods: Using immunohistochemistry and in situ hybridization, we analyzed the expression of three human-specific biomarkers: Ku80, human mitochondria (hMito) and Alu. Results: We showed that Ku80, hMito and Alu biomarkers are broadly expressed in human tissues with no or low cross-reactivity toward rat, mouse or pig tissues. In vitro, we demonstrated that their expression is stable over time and does not change along the differentiation of human-derived induced pluripotent stem cells or human glial-restricted precursors. We tracked in vivo these cell populations after transplantation in rodent spinal cords using aforementioned biomarkers and human-specific antibodies detecting apoptotic, proliferating or neural-committed cells. Conclusion: This study assesses the human-species specificity of Ku80, hMito and Alu, and proposes useful biomarkers for characterizing human stem cells in xenotransplantation paradigms.
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