Journal
REGENERATIVE MEDICINE
Volume 8, Issue 4, Pages 437-451Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/rme.13.30
Keywords
endoneurium; endothelial cell; pericyte; perineurial cell; perineurium; regenerative medicine; Schwann cell; tissue regeneration
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Funding
- Zenkyoren (National Insurance Federation of Agricultural Cooperatives)
- Tokai University Research Aid
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Aim: To cover the large tissue deficits associated with significant loss of function following surgery, a 3D gel-patch-like nerve-vascular reconstitution system was developed using the skeletal muscle-derived multipotent stem cell (Sk-MSC) sheet pellet. Materials & methods: The Sk-MSC sheet pellet was prepared from GFP transgenic mice by the collagenase extraction and 7 days expansion cell culture, and transplanted into a severe muscle damage model with large disruptions to muscle fibers, blood vessels and peripheral nerves. Results: At 4 weeks after transplantation, engrafted cells contributed to nerve-vascular regeneration associated with cellular differentiation into Schwann cells, perineurial/endoneurial cells, vascular endothelial cells and pericytes. However, skeletal myogenic differentiation was scarcely observed. Paracrine effects regarding donor cells/tissues could also be expected, because of the active expression of neurogenic and vasculogenic factor mRNAs in the sheet pellet. Conclusion: These results indicate that the vigorous skeletal myogenic potential of Sk-MSCs was clearly reduced in the sheet pellet preparation and this method may be a useful adjuvant for nerve-vascular regeneration in various tissue engineering applications.
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