Journal
REDOX REPORT
Volume 18, Issue 6, Pages 210-218Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1179/1351000213Y.0000000066
Keywords
N-acetylcysteine; Obesity proteins; Adipose differentiation
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Funding
- Universidad de Buenos Aires, Argentina [UBACYT 20020110200187, OAT 17/11]
- Ministerio de Ciencia e Innovacion Tecnologica, Espana [SAF-2009-10461]
- CONICET (Ministerio de Ciencia y Tecnica, Argentina)
- Universidad de Buenos Aires
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Objectives: Oxidative stress plays critical roles in the pathogeneses of diabetes, hypertension, and atherosclerosis, but its effect on fat accumulation is still unclear. In this study, we analyzed the role of the well-known antioxidant and a glutathione (GSH) precursor N-acetylcysteine (NAC) in fat accumulation and the expression of obesity-associated proteins. Methods: We studied the effects of 10 mu M NAC on obesity-related protein expression in cultured 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids. Results: NAC treatment inhibited fat accumulation and reduced the expression of obesity-related proteins, including monoamine oxidase A, heat shock protein 70 (HSP70), aminoacylase -1 (ACY-1), and transketolase. Discussion: Our results suggest that the effects of NAC on triglycerides (Tgs) and protein expression are correlated. In support of this, we showed that NAC treatment affected both the Tg synthesis pathway and the expression levels of proteins implicated in human obesity.
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