Journal
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Volume 25, Issue 22, Pages 3382-3386Publisher
WILEY-BLACKWELL
DOI: 10.1002/rcm.5238
Keywords
-
Funding
- Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences, United States Department of Energy
- AB SCIEX [CRADA NFE-10-02966]
- U.S. Department of Energy [DE-AC05-00OR22725]
- U.S. Government [DE-AC05-00OR22725]
Ask authors/readers for more resources
The direct separation of isomeric glucuronide metabolites from propranolol dosed tissue extracts by differential mobility spectrometry - mass spectrometry (DMS-MS) with the use of the polar gas-phase chemical modifier acetonitrile was demonstrated. The DMS gas-phase separation was able to resolve the isomeric metabolites with separation times on the order of milliseconds instead of minutes which is typically required when using pre-ionization chromatographic separation methods. Direct separation of isomeric metabolites from the complex tissue extract was confirmed by implementing a high-performance liquid chromatography (HPLC) separation prior to the DMS-MS analysis to pre-separate the species of interest. The ability to separate isomeric exogenous metabolites directly from a complex tissue extract is expected to facilitate the drug development process by increasing analytical throughput without the requirement for pre-ionization cleanup or separation strategies. Published in 2011 by John Wiley & Sons, Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available