Journal
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Volume 24, Issue 1, Pages 85-94Publisher
WILEY
DOI: 10.1002/rcm.4354
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Funding
- Genomics Research Center, Academia Sinica
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A series of saccharides, including maltoheptose, blood type B antigen, pullulan and the glucan of Ganoderma lucidum, are easily converted into the naphthimidazole (NAIM) derivatives in high yields by the iodine-promoted oxidative condensation. The NAIM-labeled saccharides, without further purification, show enhanced signals in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). The combined use of NAIM derivatization and MALDI-TOFMS analysis thus provides a rapid method for identification of saccharides even in less than 1 pmol of saccharide in the sample. Characterization of the biologically active saccharides and complex polysaccharides is also achieved through the NAIM-derivatization method. This study can be further applied to facilitate the isolation and analysis of novel saccharides. Copyright (C) 2009 John Wiley & Sons, Ltd.
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