Journal
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Volume 24, Issue 11, Pages 1507-1513Publisher
WILEY
DOI: 10.1002/rcm.4556
Keywords
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Funding
- Japan Science and Technology Agency (JST)
- Japan Society for the Promotion of Science (JSPS)
- Grants-in-Aid for Scientific Research [20228004] Funding Source: KAKEN
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Probe electrospray ionization (PESI) is a modified version of the electrospray ionization (ESI), where the capillary for sampling and spraying is replaced by a solid needle. High tolerance to salts and direct ambient sampling are major advantages of PESI compared with conventional ESI. In this study, PESI-MS was used to monitor some biological and chemical reactions in real-time, such as acid-induced protein denaturation, hydrogen/deuterium exchange (HDX) of peptides, and Schiff base formation. By using PESI-MS, time-resolved mass spectra and ion chromatograms can be obtained reproducibly. Real-time PESI-MS monitoring can give direct and detailed information on each chemical species taking part in reactions, and this is valuable for a better understanding of the whole reaction process and for the optimization of reaction parameters. PESI-MS can be considered as a potential tool for real-time reaction monitoring due to its simplicity in instrumental setup, direct sampling with minimum sample preparation and low sample consumption. Copyright (C) 2010 John Wiley & Sons, Ltd.
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