Journal
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Volume 24, Issue 22, Pages 3357-3367Publisher
WILEY
DOI: 10.1002/rcm.4746
Keywords
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Funding
- Ministry of Education, Youth and Sports of the Czech Republic [NPV II 2B08049, MSM 6046137305, 1M6215648902, FOOD-CZ-2006-016264]
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The objective of the presented study was to develop and optimize a simple, high-throughput method for the control of 32 mycotoxins (Fusarium and Alternaria toxins, aflatoxins, ergot alkaloids, ochratoxins, and sterigmatocystin) in beer. Due to the broad range of their physicochemical properties, the sample preparation step was simplified as much as possible to avoid analyte losses. The addition of acetonitrile to beer samples enabled precipitation of abundant matrix components. The clean-up efficiency was controlled by ambient mass spectrometry employing a direct analysis in real time (DART) ion source. For determination of analytes, ultra-high-performance liquid chromatography hyphenated with high-resolution mass spectrometry utilizing an orbitrap (U-HPLC-orbitrapMS) or time-of-flight (TOFMS) technology was used. Because of significantly better detection capabilities of the orbitrap technology, the U-HPLC-orbitrapMS method was chosen as a determinative step and fully validated. To compensate matrix effects, matrix-matched calibration was employed. The lowest calibration levels for most of the target mycotoxins ranged from 1 to 8 mu g L-1 beer and the recoveries of analytes were in range from 86 to 124%. Copyright (C) 2010 John Wiley & Sons, Ltd.
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