Journal
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Volume 24, Issue 22, Pages 3230-3236Publisher
WILEY
DOI: 10.1002/rcm.4757
Keywords
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Funding
- US DOE Office of Biological and Environmental Research through the BioEnergy Science Center (BESC)
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Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) was applied to the analysis of the spatial distribution of cellulose on a cross-section of juvenile poplar (Populus deltoids) stems. Microcrystalline cellulose (MCC) was used to optimize matrix (2,5-dihydroxybenzoic acid) application and instrument parameters for the detection of low hexose oligomers, which originated from cellulose in the solid phase. A section of poplar cellulose isolated from juvenile poplar stem which consisted primarily of glucose (similar to 95%) and minor components such as xylose and lignin was used for the MALDI-IMS studies. The mass spectrum of poplar cellulose consisted of a series of evenly spaced signals having a difference of 162 m/z units, which was similar to that of MCC in linear and reflectron positive ion modes. MS images of cellulose compounds with sodium ion adducts were generated and illustrated the distribution of cellulose on the surface of the poplar stem. Copyright (C) 2010 John Wiley & Sons, Ltd.
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