Journal
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Volume 23, Issue 2, Pages 258-266Publisher
WILEY
DOI: 10.1002/rcm.3873
Keywords
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Funding
- Cancer Research UK [C325/A6691]
- Experimental Cancer Medicine Centre Network [C325/A7241]
- Medical Research Council [G0100873]
- Environmental Cancer Risk
- Nutrition and Individual Susceptibility
- European Union Network of Excellence [FOOD-CT-2005-513943]
- MRC [G0100873] Funding Source: UKRI
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Urinary 8-oxo-7,8-dihydro-2 '-deoxyguanosine (8-oxodG) represents a non-invasive biomarker for oxidative stress and may be useful for monitoring chemotherapeutic and chemopreventive interventions associated with cancer-related alterations in oxidative stress. We describe the development and validation of two separate liquid chromatography/tandem mass spectrometry (LC/MS/MS) selected reaction monitoring (SRM) methods for the determination of 8-oxodG and creatinine in both murine and human urine using stable isotope labelled internal standards. Levels of 8-oxodG were normalised to creatinine. The LC/MS/MS methods were applied to two chemoprevention studies utilising tea polyphenols in humans and TRAMP (TRansgenic Adenocarcinorna of the Mouse Prostate) mice. Patients with benign prostatic hyperplasia received 1 g/day of green tea polyphenols (GTP), 1 g/day of black tea theaflavins (BTT) or no treatment for 4 weeks. TRAMP mice received GTP (0.05% in drinking water) for 4 or 25 weeks. Prostate pathology in TRAMP mice was not affected by GTP. Levels of 8-oxodG were not altered by tea polyphenols in either mice or humans. In TRAMP mice, urinary 8-oxodG levels were elevated with increasing age (p < 0.0001) but not changed by the presence of prostate tumours. In conclusion, the LC/MS/MS SRM methods described here are ideally suited for the accurate determination of 8-oxodG and creatinine in urine samples from both clinical and pre-clinical studies. Copyright (C) 2008 John Wiley & Sons, Ltd.
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