Journal
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Volume 22, Issue 17, Pages 2723-2730Publisher
WILEY
DOI: 10.1002/rcm.3665
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Funding
- BBSRC
- BBSRC [BB/D010446/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D010446/1] Funding Source: researchfish
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The growing interest in the conversion of plant biomass into biofuels has recently highlighted the lack of analytical techniques that are able to profile the fine structures of plant cell-wall polysaccharides. Here we present a new liquid chromatography/electrospray ionisation mass spectrometry (LC/ESI-MS) platform called Oligosaccharide Quantitation using Isotope Tagging (OliQuIT) developed for profiling the oligosaccharides derived from glycosyl hydrolase digestion of polysaccharides. The method is demonstrated using different arabinoxylan-derived oligosaccharide samples, which are reductively aminated with either the light (C-12(6)) or heavy (C-13(6)) form of aniline. The complex oligosaccharide mixtures are analysed by capillary normal-phase (NP)-LC and ESI-MS. Importantly, arabinoxylan oligosaccharide isomers are separated by NP-LC and their relative abundance in different samples can be determined from the intensities of ions labeled with the different isotopes. OliQuIT will be of use in multiple applications, including screening for plant varieties with improved saccharification properties, characterizing glycosyl hydrolase specificities and analysing plant glycosyl transferase mutants. Copyright (C) 2008 John Wiley & Sons, Ltd.
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