Journal
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Volume 22, Issue 12, Pages 1912-1918Publisher
WILEY
DOI: 10.1002/rcm.3564
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Funding
- Medical Research Council [G9722968] Funding Source: Medline
- Medical Research Council [G9722968] Funding Source: researchfish
- MRC [G9722968] Funding Source: UKRI
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It was shown that coupling hydrophilic interaction chromatography (HILIC) to Orbitrap Fourier transform mass spectrometery (FT-MS) provided an excellent tool for metabolic profiling, principally due to rapid elution of lipids in advance of most metabolites entering the mass spectrometer. We used in vitro cultivated procyclic forms of the protozoan parasite Trypanosoma brucei as a source of metabolites to test the performance of the HILIC column and the mass accuracy of MS. The mass accuracy achieved fell within 2ppm for all the metabolites identified within samples. It was, for example, possible to identify the signature metabolite of the trypanosome, trypanothione, and also glutathione which were well retained by the HILIC column. By comparing trypanosomes grown in two different media we were able to clearly distinguish the samples in terms of the relative abundance of a number of metabolites using Sieve 1.1 software. Copyright (C) 2008 John Wiley & Sons, Ltd.
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