Journal
RADIOLOGIA MEDICA
Volume 119, Issue 4, Pages 269-276Publisher
SPRINGER-VERLAG ITALIA SRL
DOI: 10.1007/s11547-013-0335-2
Keywords
T cells; Gold nanoparticles; In vivo imaging
Funding
- NSFC [81271540/H1801]
- STCSM [10411953500]
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Purpose Malignant tumours develop strategies to avoid immune recognition and elimination by T cells, even in individuals with a fully functioning immune system. To explore the treatment approach of adoptive immunotherapy, we exploited T cells loaded with radiolabelled gold nanoparticles (AuNPs) to track T cells in vivo. Materials and methods Surface-modified AuNPs were radiolabelled with In-111 or Cu-64. They were then transferred into T cells via electroporation. To evaluate the effectiveness of this process, T cells loaded with In-111-radiolabelled AuNPs were injected directly into the right lung of nude mice for in vivo imaging by micro-SPECT/CT. T cells loaded with Cu-64-radiolabelled AuNPs were then injected into the tail vein of nude mice and imaged by micro-PET/CT. Results High uptake signals were observed in the right lung following the direct injection of T cells containing In-111-labelled AuNPs. Imaging showed a marked difference in the dynamic biodistribution of T cells containing Cu-64-labelled AuNPs when compared with Cu-64-labelled AuNPs alone. Conclusions This study demonstrated the feasibility of the in vivo imaging of T cells loaded with radiolabelled AuNPs.
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