Journal
RADIATION PHYSICS AND CHEMISTRY
Volume 87, Issue -, Pages 53-58Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.radphyschem.2013.02.018
Keywords
Porous hollow-fiber membrane; Dimethylamino-gamma-butyric acid; Immobilization; Solvent composition; Protein adsorption
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Dimethylamino-gamma-butyric acid (DMGABA) as an ampholite was reacted with the epoxy group of the poly-glycidyl methacrylate chain grafted onto the pore surface of a porous hollow-fiber polyethylene membrane by radiation-induced graft polymerization. DMGABA was dissolved in a mixture of dioxane and water at various dioxane volume fractions, defined by dividing the dioxane volume by the total volume. The equilibrium binding capacity (EBC) of the DMGABA-immobilized porous hollow-fiber membrane for lysozyme was evaluated in the permeation mode. The EBC was varied from a 1/50-fold monolayer binding capacity to a 10-fold monolayer binding capacity by controlling the composition of the solvent used for DMGABA immobilization and the molar conversion of the epoxy group into the DMGABA group. (c) 2013 Elsevier Ltd. All rights reserved.
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