Journal
RADIATION PHYSICS AND CHEMISTRY
Volume 78, Issue 2, Pages 147-152Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.radphyschem.2008.08.010
Keywords
Bacillus flexus; Gamma irradiation; Polyhydroxyalkanoate copolymer; Polyhydroxybutyrate; FTIR spectrum; H-1 NMR spectrum
Funding
- The Department of Biotechnology, Ministry of Science and Technology, Government
- Council of Scientific and Industrial Research, India
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Bacillus flexus cultivated on sucrose and sucrose with plant oil such as castor oil produced polyhydroxybutyrate (PHB), a homopolymer of polyhydroxyalkanoate (PHA) and PHA copolymer (containing hydroxybutyrate and hexanoate), respectively. Gamma irradiation of these cells (5-40 kGy) resulted in cell damage and aided in the isolation of 45% and 54% PHA on biomass weight, correspondingly. Molecular weight of PHB increased from 1.5 X 10(5) to 1.9 x 10(5) after irradiation (10 kGy), with marginal increase of tensile strength from 18 to 20 MPa. At the same irradiation dosage, PHA copolymer showed higher molecular weight increase from 1.7 X 10(5) to 2.3 x 10(5) and tensile strength from 20 to 35 MPa. GC, GC-MS, FTIR and H-1 NMR were used for the characterization of PHA. Gamma irradiation seems to be a novel technique, to induce cross-linking and molecular weight increase of PHA copolymer and aid in easy extractability of intracellular PHA, simultaneously. (C) 2008 Elsevier Ltd. All rights reserved.
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