Journal
QUIMICA NOVA
Volume 32, Issue 1, Pages 169-174Publisher
SOC BRASILEIRA QUIMICA
DOI: 10.1590/S0100-40422009000100032
Keywords
oxidative stress; MDA; methodologies
Categories
Funding
- CNPq
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Free radicals induce lipid peroxidation, playing an important role in pathological processes. The injury mediated by free radicals can be measured by conjugated dienes, malondialdehyde, 4-hydroxynonenal, and others. However, malondialdehyde has been pointed out as the main product to evaluate lipid peroxidation. Most assays determine malondialdehyde by its reaction with thiobarbituric acid, which can be measured by indirect (spectrometry) and direct methodologies (chromatography). Though there is some controversy among the methodologies, the selective HPLC-based assays provide a more reliable lipid peroxidation measure. This review describes significant aspects about MDA determination, its importance in pathologies and biological samples treatment.
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