Journal
QUIMICA NOVA
Volume 32, Issue 8, Pages 2011-U38Publisher
SOC BRASILEIRA QUIMICA
DOI: 10.1590/S0100-40422009000800006
Keywords
antifungal activity; diterpenes; Colletotrichum
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Funding
- DIME (Direccion Nacional de Investigaciones de la Universidad Nacional de Colombia-Sede Medellin) [030802735]
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ANTIFUNGAL ACTIVITY AGAINST POSTHARVEST FUNGI BY EXTRACTS FROM COLOMBIAN PROPOLIS. The aims of the present study were to evaluate the antifungal properties of Colombian propolis extracts against Colletotrichum gloeosporioides and Botryodiplodia theobromae, and to isolate and identify the main constituents from the active extracts. Therefore, propolis samples were thoroughly extracted with n-hexane/methanol (EPEM), dichloromethane, ethyl acetate, and methanol. Experimental results indicated that mycelial growth of all selected microorganisms was reduced in culture media containing EPEM and dichloromethane fractions. Furthermore, through antifungal bioassay-guided fractionation, three known labdane-type diterpenes: isocupressic acid (1), (+)-agathadiol (2) and epi-13-torulosol (3) were isolated as the main constituents from the active fractions.
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