Journal
QUARTERLY REVIEWS OF BIOPHYSICS
Volume 42, Issue 3, Pages 139-158Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0033583509990059
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Funding
- HHMI [NTH R37 GM29169]
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This essay gives the autho's personal account on the development of concepts underlying single-particle reconstruction, a technique in electron microscopy of macromolecular assemblies with a remarkable record of achievements as of late. The ribosome proved to be an ideal testing ground for the development of specimen preparation methods, cryo-EM techniques, and algorithms, with discoveries along the way as a rich reward Increasingly. cryo-EM and single-particle reconstruction, in combination with classification techniques, is revealing dynamic information on functional molecular machines uninhibited by molecular contacts.
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