Journal
PURINERGIC SIGNALLING
Volume 5, Issue 3, Pages 299-307Publisher
SPRINGER
DOI: 10.1007/s11302-009-9143-5
Keywords
Ependymal; Cerebrospinal fluid; Inflammation; ATP; Stem cell; Subventricular zone; Neurogenesis; P2X; Purinergic
Categories
Funding
- National Institute of Health [R01 NS048256, DC007681, 2T32HL007974-05]
Ask authors/readers for more resources
Ependymal cells line the cerebral ventricles and are located in an ideal position to detect central nervous system injury and inflammation. The signaling mechanisms of ependymal cells, however, are poorly understood. As extracellular adenosine 5'-triphosphate is elevated in the context of cellular damage, experiments were conducted to determine whether ependymal cells along the mouse subventricular zone (SVZ) express functional purinergic receptors. Using whole-cell patch clamp recording, widespread expression of P2X(7) receptors was detected on ependymal cells based on their antagonist sensitivity profile and absence of response in P2X(7) (-/-) mice. Immunocytochemistry confirmed the expression of P2X(7) receptors, and electron microscopy demonstrated that P2X(7) receptors are expressed on both cilia and microvilli. Ca2+ imaging showed that P2X(7) receptors expressed on cilia are indeed functional. As ependymal cells are believed to function as partner cells in the SVZ neurogenic niche, P2X(7) receptors may play a role in neural progenitor response to injury and inflammation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available