Journal
PURE AND APPLIED CHEMISTRY
Volume 85, Issue 3, Pages 589-608Publisher
WALTER DE GRUYTER GMBH
DOI: 10.1351/PAC-REP-11-11-12
Keywords
anisotropy; fluorescence; IUPAC Analytical Chemistry Division; IUPAC Organic and Biomolecular Chemistry Division; IUPAC Physical and Biophysical Chemistry Division; photoluminescence; polarization; standardization
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Funding
- Spanish Ministry of Science and Innovation [2010CTQ16457]
- (BELSPO) Functional Supramolecular Systems (MvdV) [IAP P6/27]
- IUPAC Physical and Biophysical Chemistry Division
- IUPAC Organic and Biomolecular Chemistry Division
- IUPAC Analytical Chemistry Division
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After recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample properties. A list of selected reference values of steady-state fluorescence anisotropy and polarization is given. Attention is also paid to analysis of time-resolved fluorescence anisotropy data obtained by pulse fluorometry or phase and modulation fluorometry techniques. Recommendations for checking the accuracy of measurements are provided together with a list of selected time-resolved fluorescence anisotropy data as reported in the literature.
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