Journal
PULMONARY PHARMACOLOGY & THERAPEUTICS
Volume 22, Issue 6, Pages 483-486Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pupt.2009.04.004
Keywords
Macrolide; Quorum-sensing; DNA microarray; Pseudomonas aeruginosa
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Funding
- Ministry of Education, Culture, Sports, Science and Technology, Japan
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Low-dose macrolides are effective therapy in patients with chronic lung infections, but the mechanisms of action are unclear. In this study, we performed DNA microarray analysis of Pseudomonas aeruginosa after treatment with a low concentration of azithromycin. We found that a sub-MIC of azithromycin didn't change mRNA expression of quorum-sensing related genes (lasI, lasR, rhlI, rhlR, vft, rsaL), but lowered expression of most N-aryl homoserine lactone (AHL) synthesis enzymes upstream of last and rhlI. We propose that small down regulation of these enzymes cumulatively resulted in a larger decrease of AHL production and inhibition of quorum-sensing in P. aeruginosa. (c) 2009 Elsevier Ltd. All rights reserved.
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