Journal
BIOMEDICAL OPTICS EXPRESS
Volume 6, Issue 2, Pages 324-346Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.6.000324
Keywords
-
Funding
- UK Engineering and Physical Sciences Research Council (EPSRC) [EP/I02770X/1]
- Wellcome Trust [WT092852]
- BHF Intermediate Research Fellowship [FS/11/67/28954]
- NIHR Cardiovascular Biomedical Research Unit, Royal Brompton Hospital
- ElectroCardioMaths Programme of the BHF Centre [RG/10/11/28457]
- BBSRC [BB/E000495/1] Funding Source: UKRI
- EPSRC [EP/F040202/1, EP/I02770X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/E000495/1] Funding Source: researchfish
- British Heart Foundation [FS/11/67/28954, RG/10/11/28457] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/F040202/1, EP/I02770X/1] Funding Source: researchfish
Ask authors/readers for more resources
We investigate the potential of an instrument combining time-resolved spectrofluorometry and diffuse reflectance spectroscopy to measure structural and metabolic changes in cardiac tissue in vivo in a 16 week post-myocardial infarction heart failure model in rats. In the scar region, we observed changes in the fluorescence signal that can be explained by increased collagen content, which is in good agreement with histology. In areas remote from the scar tissue, we measured changes in the fluorescence signal (p < 0.001) that cannot be explained by differences in collagen content and we attribute this to altered metabolism within the myocardium. A linear discriminant analysis algorithm was applied to the measurements to predict the tissue disease state. When we combine all measurements, our results reveal high diagnostic accuracy in the infarcted area (100%) and border zone (94.44%) as well as in remote regions from the scar (> 77%). Overall, our results demonstrate the potential of our instrument to characterize structural and metabolic changes in a failing heart in vivo without using exogenous labels. (C) 2015 Optical Society of America
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available