Journal
BIOMEDICAL OPTICS EXPRESS
Volume 6, Issue 3, Pages 807-814Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.6.000807
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Funding
- National Institutes of Health [1R01 AI079347]
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We present a simplified approach for imaging a linear diode bar laser for application as an optical stretcher within a microfluidic geometry. We have recently shown that these linear sources can be used to measure cell mechanical properties; however, the source geometry creates imaging challenges. To minimize intensity losses and simplify implementation within microfluidic systems without the use of expensive objectives, we combine aspheric and cylindrical lenses to create a 1:1 image of the source at the stretcher focal plane and demonstrate effectiveness by measuring the deformation of human red blood cells and neutrophils. (C) 2015 Optical Society of America
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