Journal
PROTEOMICS CLINICAL APPLICATIONS
Volume 3, Issue 8, Pages 932-946Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/prca.200800111
Keywords
Absolute quantitation; MS; Plasma; Relative quantitation; Serum
Funding
- European Union [LSHG-CT-2005-512044]
- Polish Ministry of Education and Science [R13 010 03, 1/0-PBZ-MNiI-2/1/2005]
Ask authors/readers for more resources
Mounting evidence indicates that MS analysis of the human blood peptidome allows to distinguish between cancer and non-cancer samples, giving promise for a new MS-based diagnostic tool. However, several aspects of already published work have been criticized and demand for more methodical approach has been formulated. Motivated by this we undertook a systematic study of the plasma and serum peptidome using an integrated ESI-LC-MS-based platform, equipped with new data analysis tools for relative and absolute peptide quantitation. We used a high resolution LC-ESI-MS to analyze well-separated MS signals corresponding to peptides, and measured the variability of > 1000 peptide signal amplitudes across a set of plasma and serum samples from healthy individuals. By spiking serum samples with known amounts of isotopically labeled versions of a selected set of peptides we measured the variability of their absolute concentration in this sample set and demonstrated a strong influence of clotting time on the concentration of these peptides in serum. Finally, we used this new LC-ESI-MS analytical platform for the differential analysis of healthy versus colon cancer serum samples and found that it was possible to distinguish the two groups with 89.8% sensitivity and 94.6% specificity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available