Journal
PROTEOMICS
Volume 15, Issue 2-3, Pages 188-202Publisher
WILEY
DOI: 10.1002/pmic.201400147
Keywords
Affinity purification; Animal proteomics; Hippo signaling; Mass spectrometry; Protein-protein interaction; Signaling pathways
Funding
- Cancer Prevention and Research Institute of Texas
- University of Texas MD Anderson Cancer Center
- U.S. Department of Defense [W81XWH-09-1-0409]
- National Institutes of Health [CA016672]
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Signal transductions are the basis of biological activities in all living organisms. Studying the signaling pathways, especially under physiological conditions, has become one of the most important facets of modern biological research. During the last decade, MS has been used extensively in biological research and is proven to be effective in addressing important biological questions. Here, we review the current progress in the understanding of signaling networks using MS approaches. We will focus on studies of protein-protein interactions that use affinity purification followed by MS approach. We discuss obstacles to affinity purification, data processing, functional validation, and identification of transient interactions and provide potential solutions for pathway-specific proteomics analysis, which we hope one day will lead to a comprehensive understanding of signaling networks in humans.
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