Journal
PROTEOMICS
Volume 14, Issue 10, Pages 1290-1301Publisher
WILEY
DOI: 10.1002/pmic.201300329
Keywords
Biomarkers; Biomedicine; Glioblastoma multiforme; MALDI imaging MS; MALDI in-source decay; Top down
Funding
- canceropole PACA
- region PACA
- Aix Marseille Universite INSERM
- IBISA
- SIRIC [INCa-DGOS-Inserm 6038]
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Glioblastoma multiforme is one of the most common intracranial tumors encountered in adults. This tumor of very poor prognosis is associated with a median survival rate of approximately 14 months. One of the major issues to better understand the biology of these tumors and to optimize the therapy is to obtain the molecular structure of glioblastoma. MALDI molecular imaging enables location of molecules in tissues without labeling. However, molecular identification in situ is not an easy task. In this paper, we used MALDI imaging coupled to in-source decay to characterize markers of this pathology. We provided MALDI molecular images up to 30m spatial resolution of mouse brain tissue sections. MALDI images showed the heterogeneity of the glioblastoma. In the various zones and at various development stages of the tumor, using our top-down strategy, we identified several proteins. These proteins play key roles in tumorigenesis. Particular attention was given to the necrotic area with characterization of hemorrhage, one of the most important poor prognosis factors in glioblastoma.
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