Journal
PROTEOMICS
Volume 13, Issue 9, Pages 1513-1518Publisher
WILEY-BLACKWELL
DOI: 10.1002/pmic.201100659
Keywords
2DE; Aflatoxin; Aspergillus flavus; Microbiology; Proteome
Funding
- Mississippi Corn Promotion Board
- MAFES Special Research Initiative
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The filamentous fungus Aspergillus flavus is an opportunistic soil-borne pathogen that produces aflatoxins, the most potent naturally occurring carcinogenic compounds known. This work represents the first gel-based profiling analysis of A. flavus proteome and establishes a 2D proteome map. Using 2DE and MALDI-TOF-MS/MS, we identified 538 mycelial proteins of the aflatoxigenic strain NRRL 3357, the majority of which were functionally annotated as related to various cellular metabolic and biosynthetic processes. Additionally, a few enzymes from the aflatoxin synthesis pathway were also identified.
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