Journal
PROTEOMICS
Volume 13, Issue 20, Pages 2984-2988Publisher
WILEY-BLACKWELL
DOI: 10.1002/pmic.201300091
Keywords
Cell biology; High pH reversed-phase fractionation; Microglia; Protein profile; Technology
Funding
- National Research Foundation of Korea
- Korea government [MSIP] [2011-0030740]
- Proteogenomic Research Program through the National Research Foundation of Korea
- Korean Ministry of Education, Science, and Technology
- National Research Foundation of Korea [2011-0030740, 2012M3A9B9036669] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Microglia are major immune cells in the central nervous system. A characterization of microglia proteome would facilitate on the study of microglial functions in association with various neurodegenerative diseases. To build a reference proteome, we established a BV-2 microglial proteome to a depth of 5494 unique protein groups using a novel strategy that combined FASP, StageTip-based high pH fractionation, and high-resolution MS quickly and cost efficiently. By bioinformatics analysis, the BV-2 proteome is a valuable resource for studies of microglial function, such as in the immune response, inflammatory response, and phagocytosis. All MS data have been deposited in the ProteomeXchange with identifier PXD000168.
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