Journal
PROTEOMICS
Volume 12, Issue 22, Pages 3365-3373Publisher
WILEY-BLACKWELL
DOI: 10.1002/pmic.201200160
Keywords
AMT; Plant proteomics; Root hair; Soybean
Funding
- United Soybean Board
- National Science Foundation [DBI-0421620]
- Department of Energy's Office of Biological and Environmental Research
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1025752] Funding Source: National Science Foundation
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Root hairs (RH) are a terminally differentiated single cell type, mainly involved in water and nutrient uptake from the soil. The soybean RH cell represents an excellent model for the study of single cell systems biology. In this study, we identified 5702 proteins, with at least two peptides, from soybean RH using an accurate mass and time tag approach, establishing a comprehensive proteome reference map of this single cell type. We also showed that trypsin is the most appropriate enzyme for soybean proteomic studies by performing an in silico digestion of the soybean proteome using different proteases. Although the majority of proteins identified in this study are involved in basal metabolism, the function of others are more related to RH formation/function and include proteins involved in nutrient uptake (transporters) or vesicular trafficking (cytoskeleton and ras-associated binding proteins). Interestingly, some of these proteins appear to be specifically detected in RH and constitute promising candidates for further studies to elucidate unique features of this single-cell model.
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