Proteomic analysis of the microenvironment of developing oocytes

We utilized a setup based on extensive pre-fractionation of proteolytic peptides and nanoflow reversed-phase LC-MS/MS to identify the (sub) proteome of human follicular fluid (FF). In this in-depth screen, 246 specific proteins were identified, the majority of which are involved in coagulation-and immune-response pathways. Our aim is to define a set of FF protein-markers, which could predict oocyte quality.