Journal
PROTEOMICS
Volume 12, Issue 9, Pages 1463-1471Publisher
WILEY-BLACKWELL
DOI: 10.1002/pmic.201100240
Keywords
Biomedicine; Follicular fluid proteome; IEF; Nanoflow LC-MS/MS; Oocyte quality
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We utilized a setup based on extensive pre-fractionation of proteolytic peptides and nanoflow reversed-phase LC-MS/MS to identify the (sub) proteome of human follicular fluid (FF). In this in-depth screen, 246 specific proteins were identified, the majority of which are involved in coagulation-and immune-response pathways. Our aim is to define a set of FF protein-markers, which could predict oocyte quality.
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