Journal
PROTEOMICS
Volume 11, Issue 6, Pages 1099-1109Publisher
WILEY
DOI: 10.1002/pmic.201000562
Keywords
Glycosylation; Label-free; Phosporylation; Quality control; Quantification; Technology
Funding
- Ministerium fur Innovation, Wissenschaft, Forschung und Technologie des Landes Nordrhein-Westfalen
- Bundesministerium fur Bildung und Forschung [31P5800]
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Mass spectrometric characterization of protein modifications is usually based on single peptides. With the advent of large-scale PTM-focussed MS studies, vast amounts of data are generated continuously, providing biologists extremely valuable and virtually never-ending sources for targeted functional research. However, even more than for proteomics in general, appropriate strategies for quality control of the different steps of the analytical strategy are imperative to prevent functional researchers from doing Sisyphos work on false-positive and unconfident PTM assignments. Here, we describe strategies to address the important issue of quality control for PTM analysis on various levels of the analytical pipeline: sample preparation/processing, analysis/identification and finally data interpretation, for qualitative as well as quantitative studies.
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