Journal
PROTEOMICS
Volume 11, Issue 24, Pages 4726-4730Publisher
WILEY-BLACKWELL
DOI: 10.1002/pmic.201100162
Keywords
Cell Lysis; HeLa S3; MS; Protein Digestion; Technology
Funding
- German Academic Exchange service
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In order to maximize the number of proteins identified from Hela S3 cell lysate we tested various cell lysis, protein precipitation and digestion protocols. First, we compared three different lysis buffers, two mechanical cell disruption methods and two precipitation methods. Then, we tested six different in-solution digestion protocols, three different in-gel digestion protocols and ten different peptide extraction protocols. The result is a proposal for an optimized protocol to prepare the whole cell lysate samples from HeLa S3 cells.
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