Journal
PROTEOMICS
Volume 11, Issue 24, Pages 4660-+Publisher
WILEY
DOI: 10.1002/pmic.201100058
Keywords
Arthritis; Cartilage; Cell biology; Collagen fibril; Extracellular matrix; Interactions
Funding
- Arthritis Foundation
- NIH/NIAMS [RO1AR47985, KO2AR48672]
- NIH/NCRR [P20RR16454]
- National Science Foundation [0619793, 0923535]
- M. J. Murdock Foundation
- Idaho State Board of Education Higher Education Research Council
- Lori and Duane Stueckle
- St. Luke's Regional Medical Center
- Mountain States Tumor and Medical Research Institute
- Research Corporation Cottrell College
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Cartilage plays an essential role during skeletal development within the growth plate and in articular joint function. Interactions between the collagen fibrils and other extracellular matrix molecules maintain structural integrity of cartilage, orchestrate complex dynamic events during embryonic development, and help to regulate fibrillogenesis. To increase our understanding of these events, affinity chromatography and liquid chromatography/tandem mass spectrometry were used to identify proteins that interact with the collagen fibril surface via the amino terminal domain of collagen a1(XI) a protein domain that is displayed at the surface of heterotypic collagen fibrils of cartilage. Proteins extracted from fetal bovine cartilage using homogenization in high ionic strength buffer were selected based on affinity for the amino terminal noncollagenous domain of collagen a1(XI). MS was used to determine the amino acid sequence of tryptic fragments for protein identification. Extracellular matrix molecules and cellular proteins that were identified as interacting with the amino terminal domain of collagen a1(XI) directly or indirectly, included proteoglycans, collagens, and matricellular molecules, some of which also play a role in fibrillogenesis, while others are known to function in the maintenance of tissue integrity. Characterization of these molecular interactions will provide a more thorough understanding of how the extracellular matrix molecules of cartilage interact and what role collagen XI plays in the process of fibrillogenesis and maintenance of tissue integrity. Such information will aid tissue engineering and cartilage regeneration efforts to treat cartilage tissue damage and degeneration.
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