Journal
PROTEOMICS
Volume 10, Issue 12, Pages 2259-2269Publisher
WILEY
DOI: 10.1002/pmic.201000064
Keywords
Antibody; Coxiella burnetii; Humoral; Microarray; Protein arrays; Q fever
Funding
- National Institutes of Health/National Institute of Allergy and Infectious Diseases [5U01AI078213, U54065359]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [U54AI065359, U01AI078213] Funding Source: NIH RePORTER
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Comprehensive evaluation of the humoral immune response to Coxiella burnetii may identify highly needed diagnostic antigens and potential subunit vaccine candidates. Here we report the construction of a protein microarray containing 1901 C. burnetii ORFs (84% of the entire proteome). This array was probed with Q-fever patient sera and naive controls in order to discover C. burnetii-specific seroreactive antigens. Among the 21 seroreactive antigens identified, 13 were significantly more reactive in Q-fever cases than naive controls. The remaining eight antigens were cross-reactive in both C. burnetii infected and naive patient sera. An additional 64 antigens displayed variable seroreactivity in Q-fever patients, and underscore the diversity of the humoral immune response to C. burnetii. Nine of the differentially reactive antigens were validated on an alternative immunostrip platform, demonstrating proof-of-concept development of a consistent, safe, and inexpensive diagnostic assay alternative. Furthermore, we report here the identification of several new diagnostic antigens and potential subunit vaccine candidates for the highly infectious category B alphaproteobacteria, C. burnetii.
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