Journal
PROTEOMICS
Volume 10, Issue 3, Pages 555-563Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200800657
Keywords
2-DE; Deinococcus geothermalis; Microbiology; Proteome reference map
Funding
- Finnish Funding Agency for Technology and Innovation (TEKES)
- Finnish Graduate School in Environmental Science and Technology
- Biocenter Oulu
- Academy of Finland
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2-DE reference maps for Deinococcus geothermalis cytosolic and cell envelope proteomes were constructed. In total, 403 spots were identified as 299 different proteins. Unique in the proteomes were four subunits of V-type ATPase and Deinococcus specific proteins constituting one-fourth of cell envelope proteome. The cytoplasmic proteome included enzymes of the central carbon metabolism, chaperones, enzymes of protein and DNA repair, and oxidative stress. A total of 34 abundant proteins with unknown function may relate to the extreme stress tolerance of D. geothermalis.
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