Journal
PROTEOMICS
Volume 10, Issue 17, Pages 3203-3207Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.201000170
Keywords
Analysis conditions; Antibody array; Cellular protein; Protein arrays
Funding
- European Union
- PaCaNet project within the German NGFNplus program
- Deutscher Akademischer Austausch Dienst (DAAD)
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Antibody microarrays are a developing tool for global proteomic profiling. A protocol was established that permits robust analyses of protein extracts from mammalian tissues and cells rather than body fluids. The factors optimized were buffer composition for surface blocking, blocking duration, protein handling and processing, labeling parameters like type of dye, molar ratio of label versus protein, and dye removal, as well as incubation parameters such as duration, temperature, buffer, and sample agitation.
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