Journal
PROTEOMICS
Volume 10, Issue 6, Pages 1284-1296Publisher
WILEY
DOI: 10.1002/pmic.200900418
Keywords
Bioinformatics; Combined fractional diagonal chromatography; MS; Neo-N-termini; Protease substrate degradomics; Technology
Funding
- Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen)
- Fund for Scientific Research - Flanders (Belgium) [G.0077.06, G.0042.07]
- Ghent University [BOF07/GOA/012]
- Inter University Attraction Poles [IUAP06]
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Proteolytic processing has recently received increased attention in the field of signal propagation and cellular differentiation. Because of its irreversible nature, protein cleavage has been associated with committed steps in cell function. One aspect of protease biology that boomed the past few years is the detailed characterization of protease substrates by both shotgun as well as targeted MS-driven proteomics techniques. The most promising techniques are discussed in this review and we further elaborate on the bioinformatics challenges that accompany mainly qualitative, MS-driven protease substrate degradome studies.
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