Journal
PROTEOMICS
Volume 9, Issue 21, Pages 4991-4996Publisher
WILEY
DOI: 10.1002/pmic.200900065
Keywords
Atherosclerosis; Cell biology; Extracellular proteins; Protease inhibitors; Proteomic methods; Secreted proteins
Funding
- British Heart Foundation
- Oak Foundation
- British Heart Foundation [FS/08/002/24537] Funding Source: researchfish
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Human umbilical vein endothelial cells are the most widely used in vitro model for endothelial cells. Their secreted proteins, however, have not been comprehensively analysed so far. In this study, we accomplished to map the secretome of human umbilical vein endothelial cells by combining free-flow electrophoresis with nanoflow LC-MS/MS. This comprehensive analysis provides a basis for future comparative studies of protein secretion by endothelial cells in response to cardiovascular risk factors and is available on our website http://www.vascular-proteomics.com.
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