Journal
PROTEOMICS
Volume 9, Issue 22, Pages 5188-5193Publisher
WILEY-BLACKWELL
DOI: 10.1002/pmic.200900116
Keywords
4-Hydroxy-2-nonenal; Michael addition; Neutral loss-driven MS3; Protein adducts; Schiff base; Technology
Funding
- National Institutes of Health [AG025384, AA015982]
- University of North Texas Health Science Center [BK-0031]
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We report a data-dependent neutral-loss-driven MS3 acquisition to enhance, in addition to abundant Michael adducts, the detection of Schiff-base adducts of proteins and 4-hydroxy-2-nonenal, a reactive end product of lipid peroxidation. In vitro modification of cytochrome c oxidase, a mitochondrial protein complex, was used as a model to evaluate the method. The technique allowed for a confident validation of modification sites and also identified a Schiff-base adduct in subunit Vb of the protein complex.
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