Journal
PROTEOMICS
Volume 9, Issue 3, Pages 757-767Publisher
WILEY
DOI: 10.1002/pmic.200800019
Keywords
Green tea; Human lung adenocarcinoma cells; Lamins A/C; Proteomics
Funding
- NCI NIH HHS [P50 CA090388, P01 CA042710, P50 CA090388-04, P50 CA090388-020001, R01 CA090833, CA90833, CA42710, P30 CA042710, P30 CA042710-21, P50 CA090388-020004] Funding Source: Medline
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Green tea polyphenols exhibit multiple antitumor activities, and the mechanisms of action are not completely understood. Previously, we reported that green tea extract (GTE)-induced actin remolding is associated with increased cell adhesion and decreased motility in A549 lung cancer cells. To identify the cellular targets responsible for green tea-induced actin remodeling, we performed 2-DE LC-MS/MS of A549 cells before and after GTE exposure. We have identified 14 protein spots that changed in expression (>= 2-fold) after GTE treatment. These proteins are involved in calcium-binding, cytoskeleton and motility, metabolism, detoxification, or gene regulation. In particular we found upregulation of several genes that modulate actin remodeling and cell migration, including lamin A/C. Our data indicated that GTE-induced lamin A/C upregulation appears to be at the transcriptional level and the increased expression results in the decrease in cell motility, as confirmed by siRNA. The result of the study demonstrates that GTE alters the levels of many proteins involved in growth, motility and apoptosis of A549 cells and their identification may explain the multiple antitumor activities of GTE.
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