Journal
PROTEOMICS
Volume 8, Issue 9, Pages 1758-1761Publisher
WILEY
DOI: 10.1002/pmic.200700931
Keywords
frit fabrication; high resolution proteomics; liquid chromatography-tandem mass spectrometry; multiprotein complex; two-dimensional chromatography
Funding
- NCI NIH HHS [P30 CA 014089] Funding Source: Medline
- NIGMS NIH HHS [R01GM065325] Funding Source: Medline
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A modified sol-gel method for a one-step on-column frit preparation for fused-silica capillaries and its utility for peptide separation in LC-MS/MS is described. This method is inexpensive, reproducible, and does not require specialized equipments. Because the frit fabrication process does not damage polyimide coating, the frit-fabricated column can be tightly connected on-line for high pressure LC. These columns can replace any capillary liquid transfer tubing without any specialized connections up-stream of a spray tip column. Therefore multiple columns with different phases can be connected in series for one- or multiple-dimensional chromatography.
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