Journal
PROTEOMICS
Volume 8, Issue 12, Pages 2492-2499Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200700727
Keywords
malaria; MudPIT; ookinete; zygote
Funding
- NCRR NIH HHS [P41 RR11823-10, P41 RR011823] Funding Source: Medline
- NIAID NIH HHS [R21 AI053781-01, R21 AI053781-03, R21AI053781, R01 AI045999-06A2, R01AI45999, R01 AI045999, R01 AI045999-05, F32 AI062061, R21 AI053781-02, R21 AI053781, R01 AI045999-04] Funding Source: Medline
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Delineation of the complement of proteins comprising the zygote and ookinete, the early developmental stages of Plasmodium within the mosquito midgut, is fundamental to understand initial molecular parasite-vector interactions. The published proteome of Plasmodium falciparum does not include analysis of the zygote/ookinete stages, nor does that of P. berghei include the zygote stage or secreted proteins. P. gallinaceum zygote, ookinete, and ookinete-secreted/released protein samples were prepared and subjected to Multidimensional protein identification technology (MudPIT). Peptides of P. gallinaceum zygote, ookinete, and ookinete-secreted proteins were identified by MS/MS, mapped to ORFs (>50 amino acids) in the extent P. gallinaceum whole genome sequence, and then matched to homologous ORFs in P. falciparum. A total of 966 P. falciparum ORFs encoding orthologous proteins were identified; just over 40% of these predicted proteins were found to be hypothetical. A majority of putative proteins with predicted secretory signal peptides or transmembrane domains were hypothetical proteins. This analysis provides a more comprehensive view of the hitherto unknown proteome of the early mosquito midgut stages of P. falciparum. The results underpin more robust study of Plasmodium-mosquito midgut interactions, fundamental to the development of novel strategies of blocking malaria transmission.
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