Journal
PROTEOMICS
Volume 8, Issue 20, Pages 4338-4343Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200701091
Keywords
beta-Actin; beta-Tubulin; Glyceraldehyde-3-phosphate dehydrogenase; Motor neuron pathology; SOD1(G93A) animal model
Funding
- Instrumentacion y Componentes
- PROFIT Project [N 0223-2005]
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The choice of housekeeping proteins or genes for internal standards should be made carefully, taking into account the cell and tissue type, the experimental conditions, and the healthy/disease state(s) under consideration. Furthermore, as the correlation between transcriptional and translational levels of commonly used housekeeping genes is often discussed, this study shed light on the transcriptional levels of P-actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and the translational levels of beta-actin, GAPDH, and beta-tubulin in an amyotrophic lateral sclerosis mouse model.
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