Journal
PROTEOME SCIENCE
Volume 7, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1477-5956-7-16
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Funding
- Swedish Research Council
- VR
- Magnus Bergvalls Stiftelse
- Stiftelsen Langmanska kulturfonden
- Lars Hiertas Minne
- Helge Ax:son Johnsons Stifelse
- Carl Trygger Stiftelse
- Stiftelse Oscar
- Lilli Lamms Minne
- CBR
- SSF
- VINNOVA
- Wallenberg foundation
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Background: This study aims to analyze sex differences in mice aging kidney. We applied a proteomic technique based on subfractionation, and liquid chromatography coupled with 2-DE. Samples from male and female CD1-Swiss outbred mice from 28 weeks, 52 weeks, and 76 weeks were analysed by 2-DE, and selected proteins were identified by matrix assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS). Results: This proteomic analysis detected age-related changes in protein expression in 55 protein-spots, corresponding to 22 spots in males and 33 spots in females. We found a protein expression signature (PES) of aging composed by 8 spots, common for both genders. The identified proteins indicated increases in oxidative and proteolytic proteins and decreases in glycolytic proteins, and antioxidant enzymes. Conclusion: Our results provide insights into the gender differences associated to the decline of kidney function in aging. Thus, we show that proteomics can provide valuable information on age-related changes in expression levels of proteins and related modifications. This pilot study is still far from providing candidates for aging-biomarkers. However, we suggest that the analysis of these proteins could suggest mechanisms of cellular aging in kidney, and improve the kidney selection for transplantation.
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