Journal
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
Volume 83, Issue 1, Pages 1-24Publisher
WILEY
DOI: 10.1002/prot.24703
Keywords
membrane proteins; protein structure; protein modeling; sequence-based methods; structure prediction; de novo folding; homology modeling; molecular dynamics simulations; alpha-helical membrane proteins; beta-barrel membrane proteins
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Funding
- F. Stuart Hodgson Faculty Scholar Fund
- NIH [R01-GM078221]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM078221] Funding Source: NIH RePORTER
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The determination of membrane protein (MP) structures has always trailed that of soluble proteins due to difficulties in their overexpression, reconstitution into membrane mimetics, and subsequent structure determination. The percentage of MP structures in the protein databank (PDB) has been at a constant 1-2% for the last decade. In contrast, over half of all drugs target MPs, only highlighting how little we understand about drug-specific effects in the human body. To reduce this gap, researchers have attempted to predict structural features of MPs even before the first structure was experimentally elucidated. In this review, we present current computational methods to predict MP structure, starting with secondary structure prediction, prediction of trans-membrane spans, and topology. Even though these methods generate reliable predictions, challenges such as predicting kinks or precise beginnings and ends of secondary structure elements are still waiting to be addressed. We describe recent developments in the prediction of 3D structures of both -helical MPs as well as -barrels using comparative modeling techniques, de novo methods, and molecular dynamics (MD) simulations. The increase of MP structures has (1) facilitated comparative modeling due to availability of more and better templates, and (2) improved the statistics for knowledge-based scoring functions. Moreover, de novo methods have benefited from the use of correlated mutations as restraints. Finally, we outline current advances that will likely shape the field in the forthcoming decade. Proteins 2015; 83:1-24. (c) 2014 Wiley Periodicals, Inc.
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